Device
Lysis

Part:BBa_K836009:Design

Designed by: Ricardo Alvarado   Group: iGEM12_Costa_Rica-TEC-UNA   (2012-09-20)


Lysis device for R. opacus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2212
    Illegal BamHI site found at 994
    Illegal XhoI site found at 3156
    Illegal XhoI site found at 3312
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2790
    Illegal NgoMIV site found at 3048
    Illegal NgoMIV site found at 3349
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1753
    Illegal BsaI site found at 2561
    Illegal BsaI site found at 3008
    Illegal BsaI site found at 3485
    Illegal BsaI.rc site found at 410
    Illegal BsaI.rc site found at 710
    Illegal BsaI.rc site found at 983
    Illegal BsaI.rc site found at 1150
    Illegal BsaI.rc site found at 1666
    Illegal SapI site found at 1221


Design Notes

Constitutive expression of nitR and a low constitutive expression of an antiholin are required to avoid undesired lysis.


Source

The majority of the parts where made by backtranslating a protein. Others are existing biobricks while some are promoters obtained from databases as NCBI. Check each individual part to know its origin.

References